Free access
Volume 79, Number 6, 1999
Page(s) 577 - 586
Lait 79 (1999) 577-586
DOI: 10.1051/lait:1999647

Binding of benzaldehyde by β-lactoglobulin, by static headspace and high performance liquid chromatography in different physico-chemical conditions

Isabelle Andriota, Isabelle Marinb, Gilles Ferona, Perla Relkinb and Elisabeth Guicharda

a  Laboratoire de recherches sur les arômes, Inra, 17, rue Sully, 21034 Dijon cedex, France
b  Laboratoire de biophysique des matériaux alimentaires, département science de l'aliment, Ensia, 1, avenue des Olympiades, 91744 Massy cedex, France

(Received 26 January 1999; accepted 1 July 1999)

Abstract - The study of binding between β-lactoglobulin and benzaldehyde was realized using two complementary techniques: static headspace and HPLC techniques. Static headspace analysis shows global retention of benzaldehyde whereas HPLC shows the presence of strong interactions. The interactions were studied in different media (water, NaCl (50 mmol-L-1), NaCl (50 mmol-L-1) + ethanol 13 v %), with or without heat treatment. © Inra / Elsevier, Paris.

Résumé - Étude des liaisons entre le benzaldehyde et la β-lactoglobuline par headspace statique et chromatographie liquide haute pression dans des conditions physico-chimiques différentes
L'étude des interactions entre la β-lactoglobuline et le benzaldehyde est réalisée à l'aide de deux techniques complémentaires : la technique de headspace statique et la technique de CLHP. La première met en évidence une rétention globale de l'arôme tandis que la seconde montre la présence d'interactions fortes. Les interactions sont étudiées dans des milieux différents (eau, NaCl (50 mmol.L-1), NaCl (50 mmol.L-1) + ethanol 13 v %), avec ou sans traitement thermique. © Inra / Elsevier, Paris.

Key words: β-lactoglobulin / benzaldehyde / interaction / static headspace / HPLC

Mots clés : β-lactoglobuline / benzaldehyde / interaction / headspace statique / CLHP

Corresponding author: Isabelle Andriot

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