| Issue |
Lait
Volume 46, Number 451-452, 1966
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|---|---|---|
| Page(s) | 39 - 50 | |
| DOI | https://doi.org/10.1051/lait:1966451-4526 | |
DOI: 10.1051/lait:1966451-4526
Chromatographie des protéines du lactoserum de brebis
J. L. MAUBOISa, b, C. BOUILLANNEa and Barbara NISTCHKEaa Station centrale de Recherches laitières et de Technologie des Produits animaux, Centre national de Recherches zootechniques, Jouy-en-Josas (Seine-et-Oise)
b Adresse actuelle : Laboratoire de Recherches de Technologie Laitière, Centre de Recherches agronomiques de l'Ouest, Rennes, France
Abstract - Chromatography of whey proteins of ewe's milk
The separation of whey proteins of ewe's milk on D.E.A.E.-cellulose column has been studied. Twelve fractions have been detected. Their purity has been studied by paper electrophoresis. The fractions corresponding to Lactoperoxydase, Immune globulins, β-Lactoglobulin and Serum albumin have been identified by paper electrophoresis, U.V. Spectrophotometry and precipitins and anti-toxins antibiodies tests. The fractions F2 F3 and F4 contain non dialysable components with Uridine and Guanine, they are separated from the whey proteins by filtration on Sephadex G 100. The percent by weight of each fraction has been calculated and β-Lactoglobulin (fraction 8) represents 55 to 60 p. 100 of the total proteins of whey of ewe's milk