Free Access
Issue
Lait
Volume 58, Number 573-574, 1978
Page(s) 111 - 117
DOI https://doi.org/10.1051/lait:1978573-5746
Lait 58 (1978) 111-117
DOI: 10.1051/lait:1978573-5746

Différenciation rapide des colonies de colibacilles parmi les colonies de coliformes

J. JACQUET and O. COIFFIER

Laboratoire de Microbiologie, U.E.R. des Sciences de la Vie et du Comportement, 14032 Caen cedex

Abstract - A new test is proposed to differenciate E. coli from other coliforms.
Colonies from selective plates were subcultured in a peptone water for indol test and in a malonate broth, incubated at 37° C for 24 hr. The results were compared to Mackenzie, Gilbert and Taylor's for 637 strains sampled in 2 666 coliforms isolated from several types of each of the following: cheeses, milk, cream, sea-water, streams, sewage and stool specimens of calves and chickens. The particular interest of the experiment is that it allows a very precise distinction between E. coli and K. pneumoniae var. oxytoca