Growth inhibition of Enterococcus mundtii in Kefir by in situ production of bacteriocin ST8KF

Free access article

Issue		Lait Volume 86, Number 5, September-October 2006


Page(s)		401 - 405
DOI		http://dx.doi.org/10.1051/lait:2006015
Published online		12 December 2006

Lait 86 (2006) 401-405
DOI: 10.1051/lait:2006015

Note

Growth inhibition of Enterococcus mundtii in Kefir by in situ production of bacteriocin ST8KF

Jillian E. Powell¹, Svetoslav D. Todorov², Carol A. Van Reenen², Leon M.T. Dicks² and R. Corli Witthuhn¹

¹ Department of Food Science, Stellenbosch University, Private Bag X1, 7602 Matieland, South Africa
² Department of Microbiology, Stellenbosch University, Private Bag X1, 7602 Matieland, South Africa

(Received 14 June 2006 - Accepted 16 August 2006 - Published online 12 December 2006)

Abstract
Lactobacillus plantarum ST8KF, isolated from Kefir grains, produces a 3.5 kDa bacteriocin (bacST8KF) active against Enterococcus mundtii ST. Kefir produced with grains containing L. plantarum ST8KF prevented the growth of E. mundtii ST in situ. No inhibition of E. mundtii ST was recorded when Kefir was produced from grains containing a plasmid-free and bacteriocin-negative variant (ST8KF^-). Cells of E. mundtii ST were detected by fluorescent in situ hybridization (FISH). This is the first report on the incorporation of a bacteriocin-producing starter culture in Kefir grains and in situ control of microbial growth.

Résumé
Inhibition de la croissance d'Enterococcus mundtii dans le kéfir par la production in situ de la bactériocine ST8KF. Lactobacillus plantarum ST8KF, isolée de grains de kéfir, produit une bactériocine (bacST8KF) de 3,5 kDa. Dans cette étude, du kéfir a été produit avec L. plantarum ST8KF dans le but de suivre la production de la bactériocine in situ. La croissance de E. mundtii ST était inhibée dans le kéfir ainsi obtenu. Aucune inhibition n'a été observée dans les kéfirs obtenus à partir de grains contenant le variant sans plasmide et le variant bactériocine-négative (ST8KF^-). Les cellules de E. mundtii ST ont été détectées par hybridation de fluorescence in situ (FISH).

Key words: Kefir -- mass-cultured grains -- bacteriocin ST8KF -- Lactobacillus plantarum

Mots clés : kéfir -- grain cultivé en masse -- bactériocine ST8KF -- Lactobacillus plantarum

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.