Protective effect of sorbitol and monosodium glutamate during storage of freeze-dried lactic acid bacteria

Free access article

Issue		Lait Volume 83, Number 3, May-June 2003


Page(s)		203 - 210
DOI		http://dx.doi.org/10.1051/lait:2003010

Lait 83 (2003) 203-210
DOI: 10.1051/lait:2003010

Protective effect of sorbitol and monosodium glutamate during storage of freeze-dried lactic acid bacteria

Ana S. Carvalho^a, Joana Silva^a, Peter Ho^b, Paula Teixeira^a, F. Xavier Malcata^a and Paul Gibbs^a

^a Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal
^b Departamento de Ciências de Engenharia e Tecnologia, Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Apartado 574, 4901-908 Viana do Castelo, Portugal

(Received 4 July 2002; accepted 6 January 2003)

Abstract
The effects of sorbitol and monosodium glutamate upon survival during storage of freeze-dried Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus durans and Enterococcus faecalis were examined. There were no significant differences in survival during freeze-drying after addition of sorbitol or monosodium glutamate. However, these compounds were found to increase the stability of most strains during long-term storage. Various survival patterns were observed among the strains considered. However, for most of them, both compounds were proven effective in maintaining high degrees of viability over long periods of storage.

Résumé
Effet protecteur du sorbitol et du glutamate monosodique pendant le stockage de bactéries lactiques lyophilisées. Les effets du sorbitol et du glutamate monosodique sur la survie des souches lyophilisées de Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus rhamnosus, Enterococcus durans et Enterococcus faecalis pendant le stockage ont été étudiés. On n'a pas trouvé de différences significatives pendant la lyophilisation, après l'addition de sorbitol ou de glutamate monosodique. Cependant, l'addition de ces composés a augmenté la stabilité de la plupart des souches pendant le stockage prolongé. Différents profils de survie ont été observés parmi les souches considérées ; toutefois, pour la plupart des souches les deux composés étaient efficaces dans le maintien de degrés élevés de viabilité pendant de longues périodes de stockage.

Key words: Starter culture / preservation / additive / processing / viability

Mots clés : Levain / conservation / additif / traitement / viabilité

Correspondence and reprints: Paula Teixeira Tel.: +351 225580095; fax: +351 225090351;
e-mail: paula@esb.ucp.pt

© INRA, EDP Sciences 2003

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.