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Issue Lait
Volume 83, Number 2, March-April 2003
Page(s) 101 - 110
DOI 10.1051/lait:2003001

Lait 83 (2003) 101-110
DOI: 10.1051/lait:2003001

Proliferation and immunoglobulin production of hybridoma cells cultured on mastitic whey

Fatima Moussaoui, Yves Le Roux and François Laurent

Laboratoire de Sciences Animales, (Unité associée INRA), école Nationale Supérieure d'Agronomie et des Industries Alimentaires (ENSAIA), 2, avenue de la Forêt de Haye, BP 172, 54505 Vandoeuvre-Lès-Nancy, France
(Received 19 December 2001; accepted 16 September 2002)

Abstract
Lipopolysaccharide experimental mastitis was performed in order to obtain the same milk composition change involved in a clinical mastitis without, however, any bacterial proteolytic participation. Whey from milk with a high somatic cell count (SCC) had a lower mitogenic effect on anti-human prolactin-hybridoma (anti-hPL) cell culture but a greater immunostimulating effect than that of the fetal calf serum regularly used in cell culture. This immunostimulating effect was more pronounced when expressed per cell, with an effect two- to three- fold higher than that of fetal calf serum ( P < 0.01), which illustrated the efficiency of such a complement.

Résumé
Prolifération et production d'immunoglobulines d'hybridomes cultivés sur lactosérum de mammite. Une mammite expérimentale à lipopolysaccharide a été menée en vue d'obtenir le même changement de composition du lait que celui observé dans les cas de mammite clinique, en éliminant toutefois toute interaction avec la protéolyse bactérienne. Le lactosérum issu d'un lait à fort dénombrement cellulaire (DC) avait un effet mitogénique plus faible sur une culture cellulaire d'hybridomes anti-prolactine humaine (anti-hPL) mais toutefois un effet immuno-stimulant plus important que le sérum foetal de veau. Cet effet immunostimulant a été plus marqué lorsqu'il était exprimé par cellule, avec un effet 2 à 3 fois supérieur à celui observé avec le sérum de veau foetal ( P < 0,01), traduisant l'efficacité de la complémentation par un lactosérum issu d'un lait à fort DC.


Key words: Cell culture / whey / proteolysis / mastitis

Mots clés : Culture cellulaire / lactosérum / protéolyse / mammite

Correspondence and reprints: Fatima Moussaoui Tel.: (33) 3 83 59 58 89; fax (33) 3 83 59 58 04;
    e-mail: toulouse31@hotmail.com

© INRA, EDP Sciences 2003


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