FTIR spectroscopy and taxonomic purpose: Contribution to the classification of lactic acid bacteria

Free access article

Issue		Lait Volume 81, Number 1-2, January-April 2001 10^th Meeting of the " Club des Bactéries Lactiques ".


Page(s)		249 - 255
DOI		http://dx.doi.org/10.1051/lait:2001128

DOI: 10.1051/lait:2001128

Lait 81 (2001) 249-255

FTIR spectroscopy and taxonomic purpose: Contribution to the classification of lactic acid bacteria

Caroline Amiel^a, Laurence Mariey^a, Catherine Denis^b, Patricia Pichon^b and Josette Travert^a

^a Laboratoire de Physico-chimie et Biotechnologies, IUT de Caen, Université de Caen Basse Normandie, 14032 Caen Cedex, France
^b ADRIA Normandie, boulevard du 13 juin 1944, BP 2, 14310 Villers-Bocage, France

Abstract
The capacity of FTIR spectroscopy for identification and classification of lactic acid bacteria has been proved on brewery strains and more recently by ourselves on dairy strains. During our study, we underlined that IR spectra give very informative data which can be used for old or more recent taxonomical purposes. We tried to illustrate this aspect with two examples: the distinction between Streptococcus thermophilus and Streptococcus salivarius, the taxonomic range of Lactobacillus casei, paracasei, zeae and rhamnosus. The interest of this tool for taxonomic studies will be discussed.

Résumé
Apport de la spectroscopie infrarouge à transformée de Fourier en taxonomie : contribution à la classification de bactéries lactiques . La capacité de la spectroscopie IRTF pour l'identification et la classification de bactéries lactiques a été démontrée pour des souches de lactobacilles brassicoles et plus récemment par notre équipe en matière de souches laitières. Au cours de notre étude, il nous est apparu que les données spectrales obtenues, étaient riches en informations pouvant être utilisées pour étayer des discussions taxonomiques anciennes ou plus récentes. Nous illustrons notre propos par deux exemples : la distinction entre les souches types de Streptococcus thermophilus et Streptococcus salivarius et la position taxonomique des espèces Lactobacillus casei, paracasei, zeae et rhamnosus. L'intérêt et la place de cet outil en taxonomie bactérienne est présenté.

Key words: taxonomy / FTIR spectroscopy / streptococcus / lactobacillus

Mots clés : taxonomie / spectroscopie IRTF / streptococcus / lactobacillus

Correspondence and reprints: Caroline Amiel Tel.: (33) 2 31 56 71 19; fax: (33) 2 31 56 71 65;
e-mail: C.amiel@iutcaen.unicaen.fr

© INRA, EDP Sciences 2001

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