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Issue Lait
Volume 81, Number 1-2, January-April 2001
10th Meeting of the " Club des Bactéries Lactiques ".
Page(s) 105 - 114
DOI http://dx.doi.org/10.1051/lait:2001116

DOI: 10.1051/lait:2001116

Lait 81 (2001) 105-114

Analyse quantitative de la régulation de la glycolyse chez Lactococcus lactis : du gène à l'enzyme

Sergine Even, Lisa Fontaine, Nic Lindley and Muriel cocaign-bousquet

Centre de Bioingénierie Gilbert Durand, UMR 5504 INSA/CNRS et UMR 792 INSA/INRA, Institut National des Sciences Appliquées, 135 avenue de Rangueil, 31077 Toulouse Cedex 4, France

Abstract
Quantitative analysis of glycolysis regulation in Lactococcus lactis: from gene to enzyme. Transcripts of the sequenced genes encoding enzymes of central metabolism of Lactococcus lactis have been quantified using slot blot technology. Methods of quantification and data analysis were optimised to obtain a quantitative approach with high sensitivity. Transcript and enzymatic activity profiles were compared with regard to the carbon source (glucose or galactose), on two different synthetic culture media.

Résumé
Nous avons mesuré sur membranes (slot blot) les ARN messagers des gènes connus du métabolisme central de Lactococcus lactis. La méthode de mesure et d'analyse des données, optimisée pour rendre le dosage quantitatif, sera présentée ainsi que les performances du dosage. Les profils ARNm et activités enzymatiques seront comparés en fonction de la nature du substrat carboné (glucose et galactose) sur deux milieux de culture synthétiques de composition différente.


Key words: Lactococcus lactis / transcript / enzyme

Mots clés : Lactococcus lactis / transcrit / enzyme

Correspondence and reprints: Muriel cocaign-bousquet Tél. : (33) 5 61 55 94 38 ; fax : (33) 5 61 55 94 00 ; e-mail : cocaign@insa-tlse.fr

© INRA, EDP Sciences 2001


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