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Issue Lait
Volume 78, Number 1, 1998
8th Meeting of the " Club des Bactéries Lactiques ".
Page(s) 17 - 22
DOI http://dx.doi.org/10.1051/lait:199813

Lait 78 (1998) 17-22
DOI: 10.1051/lait:199813

Cométabolisme sucre-acides aminés chez Lactococcus lactis

Levente Novak, Muriel Cocaign-Bousquet, Nicholas D. Lindley and Pascal Loubière

Centre de bio-ingénierie Gilbert-Durand, UMR CNRS 5504 et laboratoire associé Inra, Institut national des sciences appliquées. Complexe scientifique de Rangueil, 31077 Toulouse cedex 4, France

Abstract - Cometabolism sugar-amino acids in Lactococcus lactis
The growth of Lactococcus lactis subsp. lactis NCDO 2118 was directly related to the medium composition as regards both the growth yield and the growth rate. The growth yield relative to the catabolic substrate, glucose, and the maximun specific growth rate increased with the medium complexity. Growth was limited by the cells' capacity to feed the anabolic pathways, while the catabolic rate was always proportional to the anabolic rate thereby avoiding energy spoilage. The conversion yield of glucose to fermentation products was constant whatever the medium composition. Approximately 88% of the glucose was recovered as classical fermentation products and. in a minimal medium containing 6 amino acids, a further 5% in biomass accounting for 60% of the total biomass. Inversely, some of the amino acids were converted to fermentation products. © Inra / Elsevier, Paris.


Résumé - La croissance de Lactococcus lactis subsp. lactis NCDO 2118 est dépendante de la composition du milieu de culture, tant en terme d'efficacité que de vitesse. Le rendement en biomasse formée par rapport au substrat catabolique, le glucose, augmente avec la richesse du milieu, de même que le taux de croissance. La croissance est limitée par les capacités de l'anabolisme, alors que le catabolisme s'adapte à l'anabolisme de façon à éviter un gaspillage d'énergie. Si la vitesse du catabolisme est d'autant plus rapide que l'anabolisme est efficace, le rendement de conversion du sucre en produits de fermentation reste constant quel que soit le milieu de culture. Sur un milieu minimal contenant six acides aminés, le glucose participe pour 88 % à la synthèse de produits de fermentation, et pour 5 % à la synthèse de biomasse, correspondant à 60 % de la biomasse formée. Inversement, les acides aminés sont à l'origine de certains produits minoritaires de fermentation. © Inra / Elsevier, Paris.


Key words: Lactococcus lactis / amino acid / growth / metabolism / energetics

Mots clés : Lactococcus lactis / acide aminé / croissance / métabolisme / énergétique




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