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Issue Lait
Volume 78, Number 1, 1998
8th Meeting of the " Club des Bactéries Lactiques ".
Page(s) 107 - 116
DOI http://dx.doi.org/10.1051/lait:1998114

Lait 78 (1998) 107-116
DOI: 10.1051/lait:1998114

Redistribution métabolique chez une souche de Lactobacillus plantarum déficiente en lactate deshydrogénase

Thierry Feraina, André N. Schanckb, Jeroen Hugenholtzc, Pascal Holsa, Willem M. de Vosd and Jean Delcoura

a  Laboratoire de génétique moléculaire, unité de génétique, université catholique de Louvain, 1348 Louvain-la-Neuve, Belgique
b  Laboratoire de chimie physique et de cristallographie, université catholique de Louvain, 1348 Louvain-la-Neuve, Belgique
c  Departments of microbiology, NIZO, Ede, Pays-Bas
d  Departments of biophysical chemistry, NIZO, Ede, Pays-Bas

Abstract - Metabolic rerouting in a Lactobacillus plantarum strain deficient for lactate dehydrogenase
We constructed a strain of Lactobacillus plantarum deficient for both D- and L-lactate dehydrogenase activities by gene inactivation. The metabolism of the resulting strain, which lacks its main fermentation pathway, was analysed by nuclear magnetic resonance. This study showed a major rerouting of both glucose and glucose / citrate metabolism which led to the production of end-products such as acetoin, ethanol, acetate, mannitol, succinate, etc. Following the heterologous expression of adh and pdc genes from Zymomonas mobilis in this strain, a part of the pyruvate flux was channeled to the production of ethanol. © Inra / Elsevier, Paris.


Résumé - Nous avons construit une souche de Lactobacillus plantarum dont les gènes codant pour les deux activités lactate deshydrogénase ont été inactivés, privant de cette manière la bactérie de sa principale voie de fermentation. L'analyse de cette souche mutante par résonance magnétique nucléaire a permis de mettre en évidence la réorientation complète du métabolisme du glucose, ainsi que du cométabolisme glucose / citrate, vers la production de composés tels que l'acétoïne, l'éthanol, l'acétate, le mannitol, le succinate... L'expression hétérologue des gènes pdc et adh de Zymomonas mobilis a provoqué chez cette souche une réorientation partielle du flux de pyruvate vers la production d'éthanol. © Inra / Elsevier, Paris.


Key words: Lactobacillus plantarum / lactate dehydrogenase / metabolism / Zymomonas mobilis / ethanol

Mots clés : Lactobacillus plantarum / lactate deshydrogénase / métabolisme / Zymomonas mobilis / ethanol




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