Purification and partial amino acid sequence of propionicin PLG-1, a bacteriocin produced by Propionibacterium thoenii P127 Purification et séquence partielle des acides aminés de la propionicine PLG-1, une bactériocine produite par Propionibacterium thoenii P127

Free access article

Issue		Lait Volume 75, Number 4-5, 1995 1^st International Symposium on Propionibacteria.


Page(s)		367 - 377
DOI		http://dx.doi.org/10.1051/lait:19954-527

Lait 75 (1995) 367-377
DOI: 10.1051/lait:19954-527

Purification and partial amino acid sequence of propionicin PLG-1, a bacteriocin produced by Propionibacterium thoenii P127

H.D. Paik^a and B.A. Glatz^{a, b}

^a Department of Food Science and Human Nutrition, lowa State University, Ames, lowa, USA
^b 2312 Food Sciences Bldg, lowa State University, Ames, IA 50011, USA

Abstract - Propionicin PLG-1, a bacteriocin produced by Propionibacterium thoenii P127, was purified to homogeneity by ammonium sulfate precipitation followed by ion exchange column chromatography and reversed-phase high-performance liquid chromatography. According to amino acid composition analysis, propionicin PLG-1 has a calculated molecular weight of 9 328 and contains 99 amino acid residues, of which 42% are hydrophobic (Ala, Ile, Leu, Val and Pro). A 10-amino acid sequence from the N-terminal end was identified: NH₂-¹Asn-²Val-³Asp-⁴Ala(Thr)-⁵Arg-⁶Thr(Cys)-⁷Ala(Thr)-⁸Arg-⁹Thr(Ala)-¹⁰Pro. No homology of this sequence to sequences of other bacteriocins from lactic acid bacteria was seen in a search of the SWISS-PROT data bank.

Résumé - Purification et séquence partielle des acides aminés de la propionicine PLG-1, une bactériocine produite par Propionibacterium thoenii P127
La propionicine PLG-1, une bactériocine produite par Propionibacterium thoenii P127, a été purifiée jusqu'à homogénéité par précipitation au sulfate d'ammonium suivie d'une chromatographie sur colonne d'échange d'ions et d'une chromatographie liquide haute-performance en phase inverse. D'après l'analyse de la composition en acides aminés, la propionicine PLG-1 a un poids moléculaire calculé de 9 328 et contient 99 résidus d'acides aminés, dont 42% sont hydrophobes (Ala, Ile, Leu, Val et Pro). Une séquence de 10 acides aminés de la partie N-terminale a été identifiée : NH₂-¹Asn-²Val-³Asp-⁴Ala(Thr)-⁵Arg-⁶Thr(Cys)-⁷Ala(Thr)-⁸Arg-⁹Thr(Ala)-¹⁰Pro. Aucune homologie de séquence avec d'autres bactériocines de bactéries lactiques n'a été trouvée en consultant la base de données SWISS-PROT.

Key words: bacteriocin / propionibacteria / propionicin / composition / purification

Mots clés : bactériocine / bactérie propionique / propionicine / séquence d'acides aminés / purification

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.