Molecular genetics of lactate-dehydrogenases from lactic acid bacteriaGénétique moléculaire des lactate-déshydrogénases des bactéries lactiques

Free access article

Issue		Lait Volume 73, Number 2, 1993 5^th Meeting of the " Club des Bactéries Lactiques ".


Page(s)		127 - 131
DOI		http://dx.doi.org/10.1051/lait:1993210

Lait 73 (1993) 127-131
DOI: 10.1051/lait:1993210

Génétique moléculaire des lactate-déshydrogénases des bactéries lactiques

J. Delcour, N. Bernard, D. Garmyn, T. Ferain and P. Hols

Unité de génétique, Université catholique de Louvain, Croix du Sud 5, 1348 Louvain-la-Neuve, Belgique

Abstract - Molecular genetics of lactate-dehydrogenases from lactic acid bacteria
We have . cloned 2 D-lactate-dehydrogenase genes (D-LDH) and one o-hydroxy-isocaproate-dehydrogenase gene (D-HicDH) from lactic acid bacteria (1...actobacillus delbrueckii subsp bulgaricus. Pediococcus acidilactici) by complementation of a LDH-negative mutant of Escherichia coli. The sequence of these genes has revealed the existence of a novel family of NADH-dependent a-hydroxy-acid dehydrogenases which were completely different from the L-LDH family. The structure-function relationships of enzymes from this new family are currently under study via site-directed mutagenesis.

Résumé - Nous avons cloné les gènes de D-lactate-déshydrogénase (D-LDH) et un gène de Dhydroxy- isocaproate-déshydrogénase (D-HicDH) de deux bactéries lactiques (Lactobacillus delbrueckii subsp bulgaricus, Pediococcus acidilacticl) par complémentation d'un mutant LDH-négatif de Escherichia coli. La séquence de ces gènes a révélé l'existence d'une famille de déshydrogénases d'a-hydroxy-acides NADH-dépendantes totalement différente de la famille des L-LDH. Les relations structure-fonction des enzymes de cette nouvelle famille sont actuellement à l'étude par mutagenèse dirigée.

Key words: lactate-dehydrogenase / hydroxy-isocaproate dehydrogenase / lactic acid bacteria / lactate / Lactobacillus bulgaricus

Mots clés : lactate-déshydrogénase / hydroxy-isocaproate déshydrogénase / bactérie lactique / acide lactique / Lactobacillus bulgaricus

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